Improved High Performance Liquid Chromatographic(HPLC)Methodfor the Analysis of Ginsenosides in Panax Ginseng Extracts & Products the Chromatographic Modifier Action of Ammonium Acetate in Separation of Ginsenosides
高效液相色谱法测定人参单体皂甙的研究──醋酸铵对人参皂甙分离的色谱改性

he chromatographic properties of ammonium acetate in HPLC mobile phase for ginsenosides assay havebeen studied, and six ginsenosides(R_(g2),T_(b1),R_c,R_d,R_(g1),R_e)were separated by the method using a column ofoctadecylsilyl chemically modified porous glass(MPG-ODS)within 15 minutes and eluted with acetonitrile-waterammonium acetate with UV detector at 204nm.The ammonium acetate in this eluant improved the separatingdegree of ginsenosides in crude drugs.This method is simple,rapid and accurate,It can be used for assaying ginseng raw materials and ginsengpreparatlons et al.