Tissue Culture and Rapid Propagation of Musella lasiocarpa, a Rare Chinese Medicinal and Ornamental Herb
珍稀药用和观赏植物地涌金莲的组织培养和快速繁殖(英文)

An efficient and rapid micropropagation system by using auxiliary buds as explants has been established for Musella lasiocarpa.Murashige and Skoog(MS) medium supplemented with 4.0 mg L-1 6-benzylaminopurine,0.2 mg L-1 1-napthaleneacetic acid,150 mg L-1 vitamin C,10% coconut milk and 3% sucrose was suitable for shoot induction and proliferation from divided buds.Each explant produced an average of 4.10 shoots after 60-day culture.After the sixth subculture,the shoot proliferation rate of plantlets was 4.23-fold.The half-strength MS medium supplemented with 1.0 mg L-1 indole-3-butyric acid and 150 mg L-1 activated charcoal was suitable for rooting.When micropropagated plantlets with well-developed root systems were transferred to planting bed containing a mixture of sand,sieved peat and perlite(1:1:1;v/v) in a greenhouse,93.5% of the plantlets survived.About 10 000 plantlets were produced successfully for field transfer after 12 months of culture initiation.This production system is useful for ex situ conservation and large-scale multiplication of M.lasiocarpa.