%0 Journal Article
%T Tissue Culture and Rapid Propagation of Musella lasiocarpa, a Rare Chinese Medicinal and Ornamental Herb<br>珍稀药用和观赏植物地涌金莲的组织培养和快速繁殖(英文)
%A ZENG Song-jun
%A WU Kun-lin
%A CHEN Zhi-lin
%A DUAN Jun
%A <br>曾宋君
%A 吴坤林
%A 陈之林
%A 段俊
%J 热带亚热带植物学报
%D 2007
%I 
%X An efficient and rapid micropropagation system by using auxiliary buds as explants has been established for Musella lasiocarpa.Murashige and Skoog(MS) medium supplemented with 4.0 mg L-1 6-benzylaminopurine,0.2 mg L-1 1-napthaleneacetic acid,150 mg L-1 vitamin C,10% coconut milk and 3% sucrose was suitable for shoot induction and proliferation from divided buds.Each explant produced an average of 4.10 shoots after 60-day culture.After the sixth subculture,the shoot proliferation rate of plantlets was 4.23-fold.The half-strength MS medium supplemented with 1.0 mg L-1 indole-3-butyric acid and 150 mg L-1 activated charcoal was suitable for rooting.When micropropagated plantlets with well-developed root systems were transferred to planting bed containing a mixture of sand,sieved peat and perlite(1:1:1;v/v) in a greenhouse,93.5% of the plantlets survived.About 10 000 plantlets were produced successfully for field transfer after 12 months of culture initiation.This production system is useful for ex situ conservation and large-scale multiplication of M.lasiocarpa.
%K Musella lasiocarpa
%K Tissue culture
%K Micropropagation<br>地涌金莲
%K 组织培养
%K 微繁殖
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=67DEF69C50E47BC4&yid=A732AF04DDA03BB3&vid=23CCDDCD68FFCC2F&iid=CA4FD0336C81A37A&sid=E514EE58E0E50ECF&eid=95D537AC89B28832&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=0&reference_num=24