The Determination of A Phenylalanine-rich Basic Protein from Plant Green Leaves Migrated towards the Cathode in SDS-PAGE
植物绿叶粗蛋白在SDS—PAGE中向负极泳动的富含苯丙氨酸的碱性蛋白的测定

Crude proteins from green leaves of Brassica campestris L.ssp.chinensis(L.) Makino var.utillis Tsen et Lee and Lactuca sativa L.were subjected to acetate cellulose membrane electrophoresis under SDS-denatured conditions.Two groups of protein were observed,one migrated towards the anode,and the other,the cathode.The cathode protein from Brassica campestris ssp.chinensis var.utillis was collected from cathode reservoir after SDS-PAGE,and its amino acid components were determined to have 57.0% phenylalanine,the ratio of basic/ acidic amino acid residues was 3.04,indicating the protein towards cathode was a phenylalanine rich basic protein.It must be emphasized that the protein migrated towards the cathode had never been reported previously.