%0 Journal Article
%T The Determination of A Phenylalanine-rich Basic Protein from Plant Green Leaves Migrated towards the Cathode in SDS-PAGE<br>植物绿叶粗蛋白在SDS—PAGE中向负极泳动的富含苯丙氨酸的碱性蛋白的测定
%A LIANG Xiu-wen
%A HUANG Li-xi
%A HUANG Cheng-gui
%A GE Jing
%A CHEN Hong-mei
%A XU Jie
%A DONG Yu-liang
%A GUO Jin-y
%A YANG Jing
%A ZENG Qiu-lian
%A XU Jie
%A <br>梁秀文
%A 黄丽
%A 黄城贵
%A 葛晶
%A 陈红梅
%A 徐杰
%A 董宇亮
%A 郭晋雅
%A 杨净
%A 曾秋莲
%A 徐杰
%J 热带亚热带植物学报
%D 2006
%I 
%X Crude proteins from green leaves of Brassica campestris L.ssp.chinensis(L.) Makino var.utillis Tsen et Lee and Lactuca sativa L.were subjected to acetate cellulose membrane electrophoresis under SDS-denatured conditions.Two groups of protein were observed,one migrated towards the anode,and the other,the cathode.The cathode protein from Brassica campestris ssp.chinensis var.utillis was collected from cathode reservoir after SDS-PAGE,and its amino acid components were determined to have 57.0% phenylalanine,the ratio of basic/ acidic amino acid residues was 3.04,indicating the protein towards cathode was a phenylalanine rich basic protein.It must be emphasized that the protein migrated towards the cathode had never been reported previously.
%K Crude protein
%K Acetate cellulose membrane electrophoresis
%K Cathode protein
%K SDS-PAGE
%K Phenylalanine<br>粗蛋白
%K 醋酸纤维薄膜电泳
%K 负极泳动蛋白
%K SDS-PAGE
%K 苯丙氨酸
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=6B3AD550F80F57EF&yid=37904DC365DD7266&vid=F3583C8E78166B9E&iid=0B39A22176CE99FB&sid=6FBD78E3BAB60869&eid=28F8B56DB6BEE30E&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=0&reference_num=9