Studies on cry1Ac Gene Transgenic Plants of Saccharum Complex by Particle Bombardment
基因枪法获得转cry1Ac基因甘蔗的研究

Plant expression vectors pUBCG0229-cry1Ac with size of 8602 bp,which had anti-insect gene and the bar gene as screening marker gene,was constructed.The result of restriction digestion showed that the construction of the vectors pUBCG0229-cry1Ac was completely correct.The embryonic callus of Saccharum Complex 'FN95-1702' and 'GT94-119' were transformed with the plasmid of pUBCG0229-cry1Ac by bombardment.After selection with PPT(Phosphinothricin) or Basta,86 regenerated plants were obtained.After detected by ...