%0 Journal Article
%T Studies on cry1Ac Gene Transgenic Plants of Saccharum Complex by Particle Bombardment<br>基因枪法获得转cry1Ac基因甘蔗的研究
%A GAO Shi-wu
%A GUO Jin-long
%A XU Li-ping
%A CHEN Ru-kai
%A FU Hua-ying
%A <br>高世武
%A 郭晋隆
%A 许莉萍
%A 陈如凯
%A 傅华英
%J 热带亚热带植物学报
%D 2011
%I 
%X Plant expression vectors pUBCG0229-cry1Ac with size of 8602 bp,which had anti-insect gene and the bar gene as screening marker gene,was constructed.The result of restriction digestion showed that the construction of the vectors pUBCG0229-cry1Ac was completely correct.The embryonic callus of Saccharum Complex 'FN95-1702' and 'GT94-119' were transformed with the plasmid of pUBCG0229-cry1Ac by bombardment.After selection with PPT(Phosphinothricin) or Basta,86 regenerated plants were obtained.After detected by ...
%K Sugarcane
%K cry1Ac gene
%K bar gene
%K Genetic transformation
%K Gene gun
%K Resistance to Chilo spp<br>甘蔗
%K cry1Ac基因
%K bar基因
%K 遗传转化
%K 基因枪
%K 抗螟虫
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=36FC7ECDB815FD058521F16873053A12&yid=9377ED8094509821&vid=2A8D03AD8076A2E3&iid=0B39A22176CE99FB&sid=E22B6B8FE86DD8F9&eid=856C2E13D1000DB7&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=0&reference_num=15