Expression of Synthetic Porcine Alpha-Lactalbumin in Transgenic Arabidopsis
人工合成的植物化猪α乳清蛋白基因在转基因拟兰芥的表达

A 398-bp plantified encoding fragment of porcine α -lactalbumin, was cloned into plasmid under the control of the cauliflower mosaic virus 35S promoter. The expression cassette was introduced into A rabidopsis with Agrobacterium mediated transformation. Some herbicide-resistant lines of transformed A rabidopsis were selected. The gene integration of chromosomal DNA level were confirmed in regenerated plants. All of them are positive for porcine α-lactalbumin gene via genomic DNA PCR. The expression at the protein level could be confirmed only in the transgenic Arabidopsis. The arabidopsis-expressed lactalbumin (LA) migrated in SDSPAGE with identical mobility to human LA prepared from human milk, indicating that the LA was correctly processed to yield a mature protein in plants