%0 Journal Article
%T Expression of Synthetic Porcine Alpha-Lactalbumin in Transgenic Arabidopsis<br>人工合成的植物化猪α乳清蛋白基因在转基因拟兰芥的表达
%A LU Chun-bin
%A <br>芦春斌
%J 热带亚热带植物学报
%D 2005
%I 
%X A 398-bp plantified encoding fragment of porcine α -lactalbumin, was cloned into plasmid under the control of the cauliflower mosaic virus 35S promoter. The expression cassette was introduced into A rabidopsis with Agrobacterium mediated transformation. Some herbicide-resistant lines of transformed A rabidopsis were selected. The gene integration of chromosomal DNA level were confirmed in regenerated plants. All of them are positive for porcine α-lactalbumin gene via genomic DNA PCR. The expression at the protein level could be confirmed only in the transgenic Arabidopsis. The arabidopsis-expressed lactalbumin (LA) migrated in SDSPAGE with identical mobility to human LA prepared from human milk, indicating that the LA was correctly processed to yield a mature protein in plants
%K Porcine α-lactalbumin
%K A rab idopsis
%K Transformation
%K Expression<br>猪α乳清蛋白
%K 拟兰芥
%K 转化
%K 蛋白质表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A74F042DF90151A4E3EF193E13FD5E1E&aid=DC2816AF3C7CEF05&yid=2DD7160C83D0ACED&vid=FC0714F8D2EB605D&iid=0B39A22176CE99FB&sid=E2546871E5B846EF&eid=4C100B7696CE9E24&journal_id=1005-3395&journal_name=热带亚热带植物学报&referenced_num=0&reference_num=15