Cloning, sequencing and expression of the coat protein gene of Cocksfoot mottle virus

The coat protein (CP) gene of Cocksfoot mottle virus (CfMV) was amplified by RT-PCR and inserted into expression vector pGEX-4T-1, and the resulting plasmid was designated as pGEXCfMV-JANCP. The fusion protein GST-CP was expressed in BL21 (DE3) pLysS after IPTG induction. The results of SDS-PAGE and Western blot analysis showed that the CfMV-CP gene was efficiently expressed in E.coli BL21 (DE3) pLysS through IPTG induction and the 56.0 kD protein was obtained.