%0 Journal Article
%T Cloning, sequencing and expression of the coat protein gene of Cocksfoot mottle virus
%A DONG Zhi
%A GUO Xiaoyu
%A ZHANG Hailong
%A ZHANG Feiyun
%A <br>DONG Zhi
%A GUO Xiaoyu
%A ZHANG Hailong
%A ZHANG Feiyun
%J 自然科学进展
%D 2007
%I 
%X The coat protein (CP) gene of Cocksfoot mottle virus (CfMV) was amplified by RT-PCR and inserted into expression vector pGEX-4T-1, and the resulting plasmid was designated as pGEXCfMV-JANCP. The fusion protein GST-CP was expressed in BL21 (DE3) pLysS after IPTG induction. The results of SDS-PAGE and Western blot analysis showed that the CfMV-CP gene was efficiently expressed in E.coli BL21 (DE3) pLysS through IPTG induction and the 56.0 kD protein was obtained.
%K Cocksfoot mottle virus
%K sequence analysis
%K prokaryotic expression<br>Cocksfoot
%K mottle
%K virus
%K sequence
%K analysis
%K prokaryotic
%K expression.
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=504AF8C1E5476CA7C4EC9DF6FEAC14AC&aid=E6D73DCBB42C467E9D5C8C3AF7208830&yid=A732AF04DDA03BB3&vid=BCA2697F357F2001&iid=0B39A22176CE99FB&sid=8B59EA573021D671&eid=4966445AEEBA9556&journal_id=1002-008X&journal_name=自然科学进展&referenced_num=0&reference_num=0