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科学通报(英文版) 2000
Effect of PKC pathway on G1 /S progression control in HeLa cells
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Abstract:
The effect of PKC activity on G1/S progression in HeLa cells has been studied. The result shows that (i) PKC activity alteration in G1 phase affects G1/S progression in HeLa cells. It has been observed that G1/S progression is stimulated by PKC agonist TPA and inhibited by PKC inhibitor GF-109203X. (ii) The expression ofc-myc andc-jun is stimulated by TPA and inhibited by GF-109203X treatment in early G1 phase. (iii) During G1/S progression, the expression of CyclinD1 is stimulated by TPA treatment and inhibited by GF-109203X treatment. There is no effect on the expression of CDK4. It is likely that PKC pathway regulates G1/S progression through regulating the expression of some early response genes and engine molecules in HeLa cells.