Sequence analysis on biological active peptides using electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometer
Sequence analysis on biological active peptides using electrospray ionizationFourier transform ion cyclotron resonance mass spectrometer

This paper describes experience with the sequencing of synthetic segment peptides of gp41 which are used in trimeric peptides as potential peptide-based vaccines and as inhibitors to mimic the fusion state of gp41 in HIV infection using ESI/CID-FT-ICR MS. All b and y′’ series ions are found in the CID spectra and the mass difference between the calculated and observed results is very slight. FT-ICR MS again shows its power in peptide sequencing, successfully helping us obtain the sequence of an unknown peptide.