Single nucleotide polymorphisms of Xylaria nigripes
黑柄炭角菌的单核苷酸多态性研究(英文)

To study the life cycle and genetic diversity of the ascomycete fungus Xylaria nigripes, single nucleotide polymorphisms (SNPs) were used as molecular marks. Based on the genomic library, a total of 193 SNPs were found in 34 fragments with 19,680bp from the two different individuals of X. nigripes, X-WC and X-LY, representing an SNP frequency of 0.981%. Among the 193 SNPs, all the sites had two alternative nucleotides, with 132 transitions and 61 transversions, resulting in a combined transition to transversion ratio of 2.16. The comparisons between the two individuals resulted in identification of 41 indels. Four of the 34 fragments were selected to examine intra-individual SNPs of the two individuals. Individual X-WC had an intra-individual SNP frequency of 1.308% (30/2293) per nucleotide and individual X-LY had a SNP frequency of 1.352% (31/2293) per nucleotide. Thirty-one sites double peak in the sequencing chromatograph indicated that the sclerotia of individual X-LY were heterokaryon.