%0 Journal Article
%T Single nucleotide polymorphisms of Xylaria nigripes<br>黑柄炭角菌的单核苷酸多态性研究(英文)
%A MA Cheng
%A CHEN Zuo-Hong
%A ZHANG Ping
%A <br>马橙
%A 陈作红
%A 张平
%J 菌物学报
%D 2011
%I 
%X To study the life cycle and genetic diversity of the ascomycete fungus Xylaria nigripes, single nucleotide polymorphisms (SNPs) were used as molecular marks. Based on the genomic library, a total of 193 SNPs were found in 34 fragments with 19,680bp from the two different individuals of X. nigripes, X-WC and X-LY, representing an SNP frequency of 0.981%. Among the 193 SNPs, all the sites had two alternative nucleotides, with 132 transitions and 61 transversions, resulting in a combined transition to transversion ratio of 2.16. The comparisons between the two individuals resulted in identification of 41 indels. Four of the 34 fragments were selected to examine intra-individual SNPs of the two individuals. Individual X-WC had an intra-individual SNP frequency of 1.308% (30/2293) per nucleotide and individual X-LY had a SNP frequency of 1.352% (31/2293) per nucleotide. Thirty-one sites double peak in the sequencing chromatograph indicated that the sclerotia of individual X-LY were heterokaryon.
%K Xylaria nigripes
%K molecular markers
%K haplotype
%K heterokaryon<br>黑柄炭角菌，分子标记，单倍型，异核体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=19FC2CE56A183C88C869B0BE23B99E97&yid=9377ED8094509821&vid=340AC2BF8E7AB4FD&iid=38B194292C032A66&sid=BA48F0B914ED890A&eid=E57FE519484CFB70&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=25