Application of CO1 for rapid identification of Pleurotus species
CO1在侧耳属物种快速鉴定中的应用

15 strains belonging to 15 species of Pleurotus were used. Primer CO332F and CO332R were designed by the cytochrome c oxidase subunit 1 gene (CO1) sequence of Pleurotus downloaded from GenBank. Results of the first-step PCR amplification showed that all the strains can get one band. The 15 strains were divided into four groups by the band size. Species-specific primers were designed accordingly. Results of the second-step PCR amplification showed that each species can get one band by the species-specific primers. In conclusion, the 15 species of Pleurotus can be identified by two-step PCR in accordance with the band size and the presence or absence of the band.