%0 Journal Article
%T Application of CO1 for rapid identification of Pleurotus species<br>CO1在侧耳属物种快速鉴定中的应用
%A SONG Chi
%A CHEN Qiang
%A XU Jing-Yu
%A ZHANG Jin-Xia
%A BIAN Yin-Bing
%A HUANG Chen-Yang
%A <br>宋驰
%A 陈强
%A 徐璟煜
%A 张金霞
%A 边银丙
%A 黄晨阳
%J 菌物学报
%D 2011
%I 
%X 15 strains belonging to 15 species of Pleurotus were used. Primer CO332F and CO332R were designed by the cytochrome c oxidase subunit 1 gene (CO1) sequence of Pleurotus downloaded from GenBank. Results of the first-step PCR amplification showed that all the strains can get one band. The 15 strains were divided into four groups by the band size. Species-specific primers were designed accordingly. Results of the second-step PCR amplification showed that each species can get one band by the species-specific primers. In conclusion, the 15 species of Pleurotus can be identified by two-step PCR in accordance with the band size and the presence or absence of the band.
%K ITS
%K two-step PCR
%K species-specific primer<br>内转录间隔区，两轮PCR，种特异引物
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=3EA1D73CA3DE3CAA53DFE5738F39B4CD&yid=9377ED8094509821&vid=340AC2BF8E7AB4FD&iid=E158A972A605785F&sid=46FF101E7ECF9F15&eid=C6FC2A9EA7E6C4B9&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=28