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菌物学报  2011 

The application of the ''bidirectional haploid nuclei migration'' in breeding and genetics of Lentinula edodes
双向核迁移在香菇遗传和育种中的应用研究

Keywords: monokaryon mating,dikaryon-monokaryon mating,Buller phenomenon
单-单杂交,双-单杂交,布勒现象

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Abstract:

Monokaryon mating tests between different strains of Lentinula edodes were carried out,and two pair dikaryotic strains were obtained by utilizing the mechanism of 'bidirectional haploid nuclei migration'.The strains were purified by isolation of single hyphal tips of dikaryotic strains and making use of 'Buller phenomenon'.Two pairs of monokaryotic and dikaryotic strains were analyzed by observations of colony shapes,microscopic examination,fruiting tests,and molecular markers.The results showed that the st...

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