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菌物学报  2012 

Can COI gene be used as DNA barcode for the nectriaceous fungi?
COI基因作为丛赤壳科真菌DNA条形码的测试

Keywords: intra-and inter-specific variation,PCR and sequencing success rate,neighbor-joining tree
种内与种间差异,PCR扩增与测序成功率,邻接树

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Abstract:

COI gene was tested with 76 strains of 33 species of the nectriaceous fungi to investigate its feasibility of serving as a DNA barcode. The results indicated that introns are present in some species, which made it difficult for PCR amplification and sequencing. COI gene is not a good DNA barcode for this group. Analysis of the obtained COI sequences showed that this gene may have the capacity to distinguish species of the nectriaceous fungi.

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