Effects of Agaricus blazei polysaccharides on the mRNA expression of cytokines in lead-poisoning rats
巴氏蘑菇多糖对铅中毒大鼠脾脏细胞因子mRNA表达的影响

To investigate the immunoregulation activity of Agaricus blazei polysaccharides on lead-poisoning rats,the effects of polysaccharides on mRNA expression of cytokines in the spleen of lead-poisoning rats were studied.Forty-eight healthy 45 aged SD rats were randomly divided into six groups(female:male=1:1),and named as control group,polysaccharide group,lead-poisoning model group,lead+50mg/kg?d polysaccharide group,lead+100mg/kg?d polysaccharide group and lead+200mg/kg?d polysaccharide group respectively.The rats of lead-poisoning model group and lead+polysaccharide group gave 0.2% lead acetate water for free drink.After treatment of 60 days,the spleens were collected,and the mRNA expressions of cytokines,such as IL-1β,IL-2,IL-6,TNF-α,IFN-γ were determined with fluorescent quantitative real-time polymerase chain reaction(FQRT-PCR).The results showed that as compared with the control group,the mRNA expression levels of IL-1β,IL-2,IL-6,TNF-α,IFN-γ of polysaccharide group and lead+200mg/kg?d polysaccharide group significantly increased at 0.05 or 0.01 level,and decreased significantly at 0.01 level in lead-poisoning model group.The mRNA expression levels of the tested cytokines in lead+200mg/kg?d polysaccharide group increased at 0.01 level as compared with the lead-poisoning model group.The expression levels were incresed with the polysaccharides increased.The result suggested that Agaricus blazei polysaccharides played an improtant role in protecting the lead-induced immune system damage of animal.