%0 Journal Article
%T Effects of Agaricus blazei polysaccharides on the mRNA expression of cytokines in lead-poisoning rats<br>巴氏蘑菇多糖对铅中毒大鼠脾脏细胞因子mRNA表达的影响
%A CHENG Hong-Yan
%A CHANG Ding-Ran
%A FENG Cui-Ping
%A CHANG Ming-Chang
%A WANG Jiang
%A LI Hai-Jie
%A LI Yue
%A <br>程红艳
%A 常鼎然
%A 冯翠萍
%A 常明昌
%A 王将
%A 李海杰
%A 李越
%J 菌物学报
%D 2012
%I 
%X To investigate the immunoregulation activity of Agaricus blazei polysaccharides on lead-poisoning rats,the effects of polysaccharides on mRNA expression of cytokines in the spleen of lead-poisoning rats were studied.Forty-eight healthy 45 aged SD rats were randomly divided into six groups(female:male=1:1),and named as control group,polysaccharide group,lead-poisoning model group,lead+50mg/kg?d polysaccharide group,lead+100mg/kg?d polysaccharide group and lead+200mg/kg?d polysaccharide group respectively.The rats of lead-poisoning model group and lead+polysaccharide group gave 0.2% lead acetate water for free drink.After treatment of 60 days,the spleens were collected,and the mRNA expressions of cytokines,such as IL-1β,IL-2,IL-6,TNF-α,IFN-γ were determined with fluorescent quantitative real-time polymerase chain reaction(FQRT-PCR).The results showed that as compared with the control group,the mRNA expression levels of IL-1β,IL-2,IL-6,TNF-α,IFN-γ of polysaccharide group and lead+200mg/kg?d polysaccharide group significantly increased at 0.05 or 0.01 level,and decreased significantly at 0.01 level in lead-poisoning model group.The mRNA expression levels of the tested cytokines in lead+200mg/kg?d polysaccharide group increased at 0.01 level as compared with the lead-poisoning model group.The expression levels were incresed with the polysaccharides increased.The result suggested that Agaricus blazei polysaccharides played an improtant role in protecting the lead-induced immune system damage of animal.
%K fluorescent quantitative real-time PCR
%K interleukin
%K tumor necrosis factor-α
%K interferon-γ<br>荧光定量
%K 白细胞介素
%K 肿瘤坏死因子α
%K γ干扰素
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=0A7F3035C3B69C9F2D3D0AA2C4D968F9&yid=99E9153A83D4CB11&vid=4AD960B5AD2D111A&iid=0B39A22176CE99FB&sid=B799C1769FCACDC8&eid=4FE459D71E3BF8EB&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=0