Isolation and identification of secondary metabolites from the solid culture of Flammulina velutipes
金针菇固体发酵菌丝体次级代谢产物分离鉴定

The ethyl acetate extracts of the solid culture of Flammulina velutipes was subjected to silica gel,ODS,sephadex LH-20 column chromatography and HPLC prepration.Four sesquiterpenoids have been isolated from the mycelial culture of this mushroom.One novel eudesmane-type sesquiterpenoid together with three known cuparene-type sesquiterpenoids were obtained,including flamvelutpenol A (1),aquaticol (2),enokipodin C (3),and limacellone (4).The absolute configuration of 1 and 4 were determined by forming complex with Rh 2 (OCOCF 3) 4 ] and then applying the bulkiness rule.Compounds 3 showed strong antibacterial activity against methicillin-resistant Staphylococcus aureus and Bacillus subtilis with MIC value 12.5mg/L and 25mg/L.Compounds 1-4 were isolated from the fungus for the first time.