%0 Journal Article
%T Isolation and identification of secondary metabolites from the solid culture of Flammulina velutipes<br>金针菇固体发酵菌丝体次级代谢产物分离鉴定
%A WANG Ya-Qi
%A YANG Xiao-Li
%A BAO Li
%A GAO Hao
%A YAO Xin-Sheng
%A LIU Hong-Wei
%A <br>王雅琪
%A 杨晓丽
%A 宝丽
%A 高昊
%A 姚新生
%A 刘宏伟
%J 菌物学报
%D 2012
%I 
%X The ethyl acetate extracts of the solid culture of Flammulina velutipes was subjected to silica gel,ODS,sephadex LH-20 column chromatography and HPLC prepration.Four sesquiterpenoids have been isolated from the mycelial culture of this mushroom.One novel eudesmane-type sesquiterpenoid together with three known cuparene-type sesquiterpenoids were obtained,including flamvelutpenol A (1),aquaticol (2),enokipodin C (3),and limacellone (4).The absolute configuration of 1 and 4 were determined by forming complex with Rh 2 (OCOCF 3) 4 ] and then applying the bulkiness rule.Compounds 3 showed strong antibacterial activity against methicillin-resistant Staphylococcus aureus and Bacillus subtilis with MIC value 12.5mg/L and 25mg/L.Compounds 1-4 were isolated from the fungus for the first time.
%K edible mushroom
%K Flammulina velutipes
%K sesquiterpenoids
%K structural elucidation
%K antimicrobial activity<br>食用菌，金针菇，倍半萜，结构鉴定，抗菌活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=3B2CD3BB807ED8FA08319BB6C715EA40&yid=99E9153A83D4CB11&vid=4AD960B5AD2D111A&iid=CA4FD0336C81A37A&sid=B344543C2864D684&eid=7C72DBC13F2D71EC&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=16