MONOCLONAL ANTIBODIES AGAINST MYCOTOXIN ZEARALENONE AND ITS APPLICATION IN IMMUNOASSAY
玉米赤霉烯酮单克隆抗体和免疫酶技术研究

Six stabilized hybridoma cell lines secreting anti-zearalenone (ZEN) monoclonal antibodies (IgG) were produced by fusing the mouse cells (SP2/0) with splenocytes isolated from Balb/c mice. The Balb/c mice were previously immunized with ZEN-bovine serum albumin (BSA) conjugation which was prepared by ZEN-oxime with BSA. An indirect competitive ELISA was used to detect ZEN by anti-ZEN McAb. Cross reactivity of these antibodies with zearalenol was 1.3-9.0%. The sensitivity of CI-ELISA for ZEN was 0.3-0.8ng/ml. Average recovery rates of ZEN spiked (25-500ng/g) corn, feed and wheat were 105%, 103% and 90%, respectively. Mean coefficients of variation were 3.8%, 15.2% and 12.7% for interwell and 5.8%, 6.8% and 2.8% for interassay, respectively.