%0 Journal Article
%T MONOCLONAL ANTIBODIES AGAINST MYCOTOXIN ZEARALENONE AND ITS APPLICATION IN IMMUNOASSAY<br>玉米赤霉烯酮单克隆抗体和免疫酶技术研究
%A Wang Jinglin Zhang Zhidong Li Zhongrun Gu Liangyuan Yin Shixing
%A <br>王景琳
%A 张志东
%A 李忠润
%A 顾良媛
%A 尹世兴
%J 菌物学报
%D 1994
%I 
%X Six stabilized hybridoma cell lines secreting anti-zearalenone (ZEN) monoclonal antibodies (IgG) were produced by fusing the mouse cells (SP2/0) with splenocytes isolated from Balb/c mice. The Balb/c mice were previously immunized with ZEN-bovine serum albumin (BSA) conjugation which was prepared by ZEN-oxime with BSA. An indirect competitive ELISA was used to detect ZEN by anti-ZEN McAb. Cross reactivity of these antibodies with zearalenol was 1.3-9.0%. The sensitivity of CI-ELISA for ZEN was 0.3-0.8ng/ml. Average recovery rates of ZEN spiked (25-500ng/g) corn, feed and wheat were 105%, 103% and 90%, respectively. Mean coefficients of variation were 3.8%, 15.2% and 12.7% for interwell and 5.8%, 6.8% and 2.8% for interassay, respectively.
%K Zearalenone
%K Monoclonal antibodies
%K Immunoassay<br>玉米赤霉烯酮
%K 单克隆抗体
%K 免疫分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=B6FD67922D884EEF0560CA86157DF643&yid=3EBE383EEA0A6494&vid=FC0714F8D2EB605D&iid=E158A972A605785F&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=0