ISOLATION OF COLD INDUCED GENES BY MODIFIED cDNA-AFLP WITH SINGLE ENZYME DIGESTION FROM VOLVARIELLA VOLVACEA
单酶切cDNA-AFLP改良法分离草菇冷诱导基因

Ten cold induced gene fragments VC7, VC8, VC9, VC10, VC11, VC12, VC13, VC14, VC15, VC16 were isolated from Volvariella volvacea by modified cDNA-AFLP with single enzyme digestion. DNA sequence results revealed that these DNA fragments had 504bp, 549bp, 555bp, 301bp, 189bp, 602bp, 605bp, 834bp, 703bp, 1085bp respectively. BLAST search results showed that VC7, VC8, VC9, VC10, VC11, VC12, VC13, VC15, VC16 had no significant homologous nucleotide sequences found in the databases yet except that VC14 was highly homologous with Neurospora crassa partial nca-1 gene for calcium P-type ATPase. Translated protein sequences of VC7, VC8, VC9, VC10, VC12, VC13, VC14, VC16 had high similarities to some corresponding protein sequences of Neurospora crassa, Schizosaccharomyces pombe and Streptomyces avermitilis, while translated protein sequences of VC11 and VC15 had no significant homologous protein sequences found in the databases yet.