%0 Journal Article
%T ISOLATION OF COLD INDUCED GENES BY MODIFIED cDNA-AFLP WITH SINGLE ENZYME DIGESTION FROM VOLVARIELLA VOLVACEA<br>单酶切cDNA-AFLP改良法分离草菇冷诱导基因
%A GUO Li-Qiong
%A LIN Jun-Fang
%A YANG Li-Qing LIU Rui-Rui
%A <br>郭丽琼
%A 林俊芳
%A 杨丽卿
%A 刘瑞瑞
%J 菌物学报
%D 2004
%I 
%X Ten cold induced gene fragments VC7, VC8, VC9, VC10, VC11, VC12, VC13, VC14, VC15, VC16 were isolated from Volvariella volvacea by modified cDNA-AFLP with single enzyme digestion. DNA sequence results revealed that these DNA fragments had 504bp, 549bp, 555bp, 301bp, 189bp, 602bp, 605bp, 834bp, 703bp, 1085bp respectively. BLAST search results showed that VC7, VC8, VC9, VC10, VC11, VC12, VC13, VC15, VC16 had no significant homologous nucleotide sequences found in the databases yet except that VC14 was highly homologous with Neurospora crassa partial nca-1 gene for calcium P-type ATPase. Translated protein sequences of VC7, VC8, VC9, VC10, VC12, VC13, VC14, VC16 had high similarities to some corresponding protein sequences of Neurospora crassa, Schizosaccharomyces pombe and Streptomyces avermitilis, while translated protein sequences of VC11 and VC15 had no significant homologous protein sequences found in the databases yet.
%K Mushroom
%K cold stress gene
%K gene isolation<br>食用菌
%K 冷胁迫基因
%K 基因分离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=70B275DE59CEAA1B&yid=D0E58B75BFD8E51C&vid=EA389574707BDED3&iid=0B39A22176CE99FB&sid=6A73B36E85DB0CE9&eid=002786F01A86D891&journal_id=1672-6472&journal_name=菌物学报&referenced_num=4&reference_num=21