Construction and Expression of Prokaryotic Expression Vector of Wheat Histone H1 Gene Conservative Sequence
小麦组蛋白H1基因保守序列原核载体的构建及表达

TAT-H1bs fusion gene was obtained through PCR amplification,and recombinant expression plasmid pET-28a-TAT-H1bs was constructed by gene operation technology.The correct recombinant expression plasmid was transformed into the host strain Rosseta induced by IPTG.The specific protein expressed was detected by SDS-PAGE and Western blot.The result showed that the targeted protein was detected in Rosseta(DE3),the fusion protein was expressed at high level amounting to 40% of the total bacterial protein analyzed b...