%0 Journal Article
%T Construction and Expression of Prokaryotic Expression Vector of Wheat Histone H1 Gene Conservative Sequence<br>小麦组蛋白H1基因保守序列原核载体的构建及表达
%A SONG Hong-xiao
%A TAN Guang-yun
%A WANG Chun-yan
%A ZHANG Rui-xing
%A ZHU Qian-kun
%A SUN Hao-ran
%A <br>宋红肖
%A 谭广云
%A 王春艳
%A 张瑞兴
%A 朱乾琨
%A 孙浩然
%J 吉林农业大学学报
%D 2010
%I 
%X TAT-H1bs fusion gene was obtained through PCR amplification,and recombinant expression plasmid pET-28a-TAT-H1bs was constructed by gene operation technology.The correct recombinant expression plasmid was transformed into the host strain Rosseta induced by IPTG.The specific protein expressed was detected by SDS-PAGE and Western blot.The result showed that the targeted protein was detected in Rosseta(DE3),the fusion protein was expressed at high level amounting to 40% of the total bacterial protein analyzed b...
%K wheat histone H1
%K fusion protein
%K soluble expression
%K cell-penetrating peptide TAT<br>小麦组蛋白H1
%K 融合蛋白
%K 可溶性表达
%K 穿膜肽TAT
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=74C612B26EF38BF0D4C997E3F9E167A1&aid=8E908C6A13DBD556200218F21DDC76D2&yid=140ECF96957D60B2&vid=9971A5E270697F23&iid=CA4FD0336C81A37A&sid=7C3A4C1EE6A45749&eid=ECE8E54D6034F642&journal_id=1000-5684&journal_name=吉林农业大学学报&referenced_num=0&reference_num=12