ASSESSMENT OF RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS IN THE CLASSIFICATION OF CANDIDA ALBICANS
随机扩增多态性对白色念珠菌分型的估价

Randomly amplified polymorphic DNA markers (RAPD-PCR) analysis-was performed in 46 clinic strains and 2 standard strains of Candida albicans. Only the profiles of OPA-14 derived from 18 random primers were very distict. It showed that the amplified numbers of band were from 6 to 12 among different strains. However, there were 6 identical fragments that can be observed in all the amplified profiles of strains, the length of fragments varied from 300 to 2 000bp. Most of strains showed a wide diversity in number and length of amplified fragments. In this paper, the feasibility of application of RAPD-PCR and isolation of fungal DNA by means of benzyl chloride in clinic and epidemiology were also discussed.