%0 Journal Article %T ASSESSMENT OF RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS IN THE CLASSIFICATION OF CANDIDA ALBICANS
随机扩增多态性对白色念珠菌分型的估价 %A Li Dongmei Wang Duanli Li Ruoyu Research Center of Medical Mycology %A the First Teaching Hospital %A Beijing Medical University Beijing Zhu Heng Zhu Lihuang Institute of Genetics %A Academia Sinica %A Beijing %A
李冬梅 %A 王瑞礼 %A 李若瑜 %A 朱衡 %A 朱立煌 %J 菌物学报 %D 1995 %I %X Randomly amplified polymorphic DNA markers (RAPD-PCR) analysis-was performed in 46 clinic strains and 2 standard strains of Candida albicans. Only the profiles of OPA-14 derived from 18 random primers were very distict. It showed that the amplified numbers of band were from 6 to 12 among different strains. However, there were 6 identical fragments that can be observed in all the amplified profiles of strains, the length of fragments varied from 300 to 2 000bp. Most of strains showed a wide diversity in number and length of amplified fragments. In this paper, the feasibility of application of RAPD-PCR and isolation of fungal DNA by means of benzyl chloride in clinic and epidemiology were also discussed. %K Candida albicans %K Classification %K Randomly amplified polymorphic DNA markers
白色念珠菌,分型,随机扩增多态性DNA %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=78CE9ACE13ACE12B35877E0EB968AC95&yid=BBCD5003575B2B5F&vid=F3583C8E78166B9E&iid=0B39A22176CE99FB&journal_id=1672-6472&journal_name=菌物学报&referenced_num=0&reference_num=0