Cloning and sequence analysis of cDNA and upstream sequence of Bbhsp70 gene from Beauveria bassiana
球孢白僵菌热休克蛋白基因Bbhsp70的cDNA及上游序列克隆与分析

The full-length cDNA of Bbhsp70 gene was cloned from Beauveria bassiana using SMART RACE RT-PCR, and its upstream sequence was amplified using genome walking. Analysis of the cloned complete cDNA with a whole length of 2405bp showed that it encompassed a open reading frame (ORF) with 1971bp encoding 656 amino acid. The mature protein had a molecular mass of 71.3kDa with a calculated pI of 4.92. The analyses indicate that the upstream sequence contained several regulatory elements, such as CCAAT-binding fact...