%0 Journal Article
%T Cloning and sequence analysis of cDNA and upstream sequence of Bbhsp70 gene from Beauveria bassiana<br>球孢白僵菌热休克蛋白基因Bbhsp70的cDNA及上游序列克隆与分析
%A XIE Ling
%A CHEN Hong-Mei
%A PU Shun-Chang
%A TANG Qiang
%A LI Zeng-Zhi
%A HUANG Bo
%A <br>谢 翎 陈红梅
%A 蒲顺昌 汤 强 李增智 黄 勃
%J 菌物学报
%D 2009
%I 
%X The full-length cDNA of Bbhsp70 gene was cloned from Beauveria bassiana using SMART RACE RT-PCR, and its upstream sequence was amplified using genome walking. Analysis of the cloned complete cDNA with a whole length of 2405bp showed that it encompassed a open reading frame (ORF) with 1971bp encoding 656 amino acid. The mature protein had a molecular mass of 71.3kDa with a calculated pI of 4.92. The analyses indicate that the upstream sequence contained several regulatory elements, such as CCAAT-binding fact...
%K RACE<br>昆虫病原真菌
%K DNA步移
%K 上游调控序列
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=CA9F815FCB0F62294C4266BB6F902ACB&aid=BB359AA77A63B97640DB7A3FDDC65FC4&yid=DE12191FBD62783C&vid=D3E34374A0D77D7F&iid=0B39A22176CE99FB&sid=E2B9962CCD971A0D&eid=334C61CAF4C8EF4E&journal_id=1672-6472&journal_name=菌物学报&referenced_num=1&reference_num=0