Isolation of high efficient biphenyl-biodegrading strain and its characteristics
高效联苯降解菌的筛选及其特性

Coking wastewater is refractory biodegraded industrial wastewater containing high concentration organics. Of the organics, polycyclic aromatic hydrocarbons (PAHs) is one of refractory biodegraded pollutants, and will hazard the environment greatly. A method of bioaugmentation for efficient PAHs biodegradation was studied. Biphenyl was a model compound selected as a unique carbon source for microbes screening. Six strains of microbes were isolated from coking wastewater and sludge by biological selective culture and multistage accumulation. WIS-01 was the most efficient one selected by the method of acclimation with a step up toxic load. Identification, growth and biphenyl-degradation of WIS-01 were studied further. Experimental results indicate that the degradation rate of WIS-01 could reach to 99% in three days. And its maximum tolerated concentration of biphenyl is 2 g/L. The colony of WIS-01 is Pseudomonas identified by 16S rDNA sequence, morphological, biochemical and physiological experiments, and it shares the homology up to 95%.