%0 Journal Article
%T Isolation of high efficient biphenyl-biodegrading strain and its characteristics<br>高效联苯降解菌的筛选及其特性
%A Wu Xiaoqin
%A Zhang Mengsi
%A Zhou Wei
%A Wei Renling
%A <br>吴晓琴
%A 张梦思
%A 周卫
%A 魏仁零
%J 环境工程学报
%D 2012
%I 
%X Coking wastewater is refractory biodegraded industrial wastewater containing high concentration organics. Of the organics, polycyclic aromatic hydrocarbons (PAHs) is one of refractory biodegraded pollutants, and will hazard the environment greatly. A method of bioaugmentation for efficient PAHs biodegradation was studied. Biphenyl was a model compound selected as a unique carbon source for microbes screening. Six strains of microbes were isolated from coking wastewater and sludge by biological selective culture and multistage accumulation. WIS-01 was the most efficient one selected by the method of acclimation with a step up toxic load. Identification, growth and biphenyl-degradation of WIS-01 were studied further. Experimental results indicate that the degradation rate of WIS-01 could reach to 99% in three days. And its maximum tolerated concentration of biphenyl is 2 g/L. The colony of WIS-01 is Pseudomonas identified by 16S rDNA sequence, morphological, biochemical and physiological experiments, and it shares the homology up to 95%.
%K biphenyl
%K bioaugmentation
%K degradation
%K screening
%K identification<br>联苯
%K 生物强化
%K 降解
%K 筛选
%K 鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=3FF3ABA7486768130C3FF830376F43B398E0C97F0FF2DD53&cid=92E6F4267FD4CBCB51B1E49E014D8054&jid=3567BD61129AA59043F5DE01F8815DB5&aid=66FD72374B2E351FC05CB7749AFFB040&yid=99E9153A83D4CB11&vid=B31275AF3241DB2D&iid=9CF7A0430CBB2DFD&sid=B68609DDFEAD78D6&eid=415CEFD1F6D0C22F&journal_id=1673-9108&journal_name=环境工程学报&referenced_num=0&reference_num=21