Study on production of a novel bioflocculant using molasses wastewater and conditions optimization and effect experiments
利用糖蜜废水生产微生物絮凝剂及条件优化和效果实验研究

The bioflocculant-producing bacteria Pseudomonas alcaligenes PS-25 was cultured in the culture medium, where the molasses wastewater replaced glucose as carbon source and energy source in the paper. The results obtained showed that the optimal culture conditions were COD concentration of 5 000 mg/L, of inoculum size of 5% (V/V) , 30 ℃, initial pH 6. 5 and shaking speed of 160 r/min, incubation time of 72 h, under the conditions, the highest flocculating activity achieved for Kaolin suspension was 96.75% . The wastewater treatment experimental results indicated that the turbidity and chroma removal rates of the bioflocculant for several wastewaters were above 90% and 80% , respectively. The COD removal rate was 73.60%～91.10% . It is concluded that the method of production of bioflocculants by culture of the PS-25 using molasses wastewater is feasible.