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海洋科学 2011
Effects of Cu2+ on Arginine kinase: activity changes, conformational changes, and aggregation
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Abstract:
Arginine kinase (AK) plays an important role in the cellular energy metabolism of invertebrate. AK from sea cucumber Stichopus japonicus is dimeric. The effects of Cu2+ on AK were studied by measuring activity changes, kinetic time course of inactivity, far-UV circular dichroism spectra, fluorescence spectra, and turbidity changes at 400nm. These results suggested that Cu2+ caused AK inactivation accompanied by conformational change, exposure of hydrophobic surface, and aggregation. The effects of Cu2+ on AK are distinctive compared with other metal divalent ions.
