%0 Journal Article
%T Effects of Cu2+ on Arginine kinase: activity changes, conformational changes, and aggregation<br>铜离子对海参精氨酸激酶活力与结构的影响
%A LIU Tao-tao
%A WANG Xi-cheng
%A <br>刘陶陶
%A 王希成
%J 海洋科学
%D 2011
%I 
%X Arginine kinase (AK) plays an important role in the cellular energy metabolism of invertebrate. AK from sea cucumber Stichopus japonicus is dimeric. The effects of Cu2+ on AK were studied by measuring activity changes, kinetic time course of inactivity, far-UV circular dichroism spectra, fluorescence spectra, and turbidity changes at 400nm. These results suggested that Cu2+ caused AK inactivation accompanied by conformational change, exposure of hydrophobic surface, and aggregation. The effects of Cu2+ on AK are distinctive compared with other metal divalent ions.
%K Arginine kinase
%K Cu2+
%K aggregation
%K unfolding<br>精氨酸激酶(Arginine
%K kinase
%K EC2.7.3.3)
%K Cu2+
%K 聚沉
%K 去折叠
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=DFE7B94EB76B8F26135C9890832CEF6A&aid=60F3B32FD1746BFDAE2A1187A23F6828&yid=9377ED8094509821&vid=6209D9E8050195F5&iid=CA4FD0336C81A37A&sid=BCA2697F357F2001&eid=659D3B06EBF534A7&journal_id=1000-3096&journal_name=海洋科学&referenced_num=0&reference_num=11