Antioxidant activity of the complexes of sulfation chitosan with metalion
壳聚糖硫酸酯金属配合物的抗氧化活性

The O_2~(·-)and ~·OH scavenging activities of the complexes of sulfation chitosan with copper(Ⅱ)or zinc(Ⅱ)and DMW chitosan were investigated.Colorimetric method was used,where phenzine methosulphate(PMS)/dinucleotide-reduced(NADH)and Fe/H_2O_2 were used as producing reagents and Nitro blue tetrazolium (NBT) and Fanhonghua as detecting reagents for O_2~(·-)and ~·OH.As expected,we obtained several satisfying resulte, as follow: Firstly,compared with DMW chitosan,the complexes of sulfation chitosan with copper(Ⅱ)or zinc (Ⅱ)had the stronger inhibition effect on O_2~(·-).For example,when mass concentration of samples was 0.025 g/L,the O_2~(·-)scavenging activity of the complexes of sulfation chitosan with copper(Ⅱ)and the complexes of sulfation chitosan with zinc(Ⅱ)were 94.18%and 93.19%respectively.Secondly,scavenging activity of the complexes of sulfation chitosan with copper(Ⅱ)or zinc(Ⅱ)(67.39%,60.46%,3.2 g/L)on ~·OH was between low molecular weight chitosan(20 ku,88.06%,3.2 g/L)and high molecular weight chitosan(761 ku,18.71%,3.2 g/L).Thirdly,low molecular weight chitosan had stronger scavenging effect on O_2~(·-)and ~·OH than high molecular weight chitosan.At 1.6 g/L,the scavenging activity of low molecular weight chitosan(20 ku)on O_2~(·-)reached 54.69%,while that of high molecular weight chitosan(761 ku)was 35.50%.Finally,the scavenging activity of several samples increased with increase in their mass concentration.However,the complexes of sulfation chitosan with copper(Ⅱ)or zinc(Ⅱ)had a quite strong scavenging action to O_2~(·-),their scavenging activity barely had obvious changing over 0.025 g/L.This result also showed that the complexes of sulfation chitosan with metalion were very effective antioxidant.