%0 Journal Article
%T Antioxidant activity of the complexes of sulfation chitosan with metalion<br>壳聚糖硫酸酯金属配合物的抗氧化活性
%A LIU Song
%A XING Rong-e
%A CAI Sheng-bao
%A YU Hua-hua
%A LI Peng-cheng
%A <br>刘松
%A 邢荣娥
%A 蔡圣宝
%A 于华华
%A 李鹏程
%J 海洋科学
%D 2009
%I 
%X The O_2~(·-)and ~·OH scavenging activities of the complexes of sulfation chitosan with copper(Ⅱ)or zinc(Ⅱ)and DMW chitosan were investigated.Colorimetric method was used,where phenzine methosulphate(PMS)/dinucleotide-reduced(NADH)and Fe/H_2O_2 were used as producing reagents and Nitro blue tetrazolium (NBT) and Fanhonghua as detecting reagents for O_2~(·-)and ~·OH.As expected,we obtained several satisfying resulte, as follow: Firstly,compared with DMW chitosan,the complexes of sulfation chitosan with copper(Ⅱ)or zinc (Ⅱ)had the stronger inhibition effect on O_2~(·-).For example,when mass concentration of samples was 0.025 g/L,the O_2~(·-)scavenging activity of the complexes of sulfation chitosan with copper(Ⅱ)and the complexes of sulfation chitosan with zinc(Ⅱ)were 94.18%and 93.19%respectively.Secondly,scavenging activity of the complexes of sulfation chitosan with copper(Ⅱ)or zinc(Ⅱ)(67.39%,60.46%,3.2 g/L)on ~·OH was between low molecular weight chitosan(20 ku,88.06%,3.2 g/L)and high molecular weight chitosan(761 ku,18.71%,3.2 g/L).Thirdly,low molecular weight chitosan had stronger scavenging effect on O_2~(·-)and ~·OH than high molecular weight chitosan.At 1.6 g/L,the scavenging activity of low molecular weight chitosan(20 ku)on O_2~(·-)reached 54.69%,while that of high molecular weight chitosan(761 ku)was 35.50%.Finally,the scavenging activity of several samples increased with increase in their mass concentration.However,the complexes of sulfation chitosan with copper(Ⅱ)or zinc(Ⅱ)had a quite strong scavenging action to O_2~(·-),their scavenging activity barely had obvious changing over 0.025 g/L.This result also showed that the complexes of sulfation chitosan with metalion were very effective antioxidant.
%K chitosan
%K the complexes of sulfation chitosan with metalion
%K superoxide radical
%K hydroxyl radical
%K antioxidant activity<br>壳聚糖
%K 壳聚糖硫酸酯金属配合物
%K 超氧阴离子自由基(O2·-)
%K 羟自由基(·OH)
%K 抗氧化活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=DFE7B94EB76B8F26135C9890832CEF6A&aid=E26CE4061F27AB6B1B9DFFDDBE81B541&yid=DE12191FBD62783C&vid=27746BCEEE58E9DC&iid=708DD6B15D2464E8&sid=708DD6B15D2464E8&eid=F3583C8E78166B9E&journal_id=1000-3096&journal_name=海洋科学&referenced_num=1&reference_num=22