The Applications of mitochondrial DNA in phylogeny reconstruction and species identification of portunid crab
线粒体基因片段在梭子蟹系统发育及物种鉴定中的应用

The partial sequences of COI and 16S genes were determined from Portunus trituberculatus,and partial sequences of COI was determined from sampled specimen.All homologous partial sequences of COI and 16S in Portunidae were collected from GenBank and aligned with the homologous sequences of P.trituberculatus.The NJ tree and MP tree were constructed using Kimura 2-parameter model.Homologous sequences(429 bp) of sampled specimen and genus Charybdis were aligned.The results show that the average content of AT is obviously higher than that of GC in both genes.Charybdis is a different group which separated from Portunus,Callinectes and Scylla.Charybdis should be included in subfamily Thalamitinae but not in subfamily Portuninae.Sampled specimen is identified as Charybdis acuta for its genetic distance and R value.This experiment researched on the phylogenetic relationships of portunid crab and identification of sampled specimen,and it provides important information for the phylogeny reconstruction and species identification of portunid crab.