%0 Journal Article
%T The Applications of mitochondrial DNA in phylogeny reconstruction and species identification of portunid crab<br>线粒体基因片段在梭子蟹系统发育及物种鉴定中的应用
%A ZHANG Shu
%A CUI Zhao-xia
%A WANG Chun-lin
%A ZHANG Shu
%A LI Xi-lian
%A CUI Zhao-xia
%A WANG Hong-xia
%A WANG Chun-lin
%A LIU Xiao-lin
%A <br>张姝
%A 李喜莲
%A 崔朝霞
%A 王鸿霞
%A 王春琳
%A 刘小林
%J 海洋科学
%D 2008
%I 
%X The partial sequences of COI and 16S genes were determined from Portunus trituberculatus,and partial sequences of COI was determined from sampled specimen.All homologous partial sequences of COI and 16S in Portunidae were collected from GenBank and aligned with the homologous sequences of P.trituberculatus.The NJ tree and MP tree were constructed using Kimura 2-parameter model.Homologous sequences(429 bp) of sampled specimen and genus Charybdis were aligned.The results show that the average content of AT is obviously higher than that of GC in both genes.Charybdis is a different group which separated from Portunus,Callinectes and Scylla.Charybdis should be included in subfamily Thalamitinae but not in subfamily Portuninae.Sampled specimen is identified as Charybdis acuta for its genetic distance and R value.This experiment researched on the phylogenetic relationships of portunid crab and identification of sampled specimen,and it provides important information for the phylogeny reconstruction and species identification of portunid crab.
%K mitochondrial DNA
%K portunid crab
%K phylogeny
%K barcode<br>线粒体基因
%K 梭子蟹
%K 系统发育
%K 分子分类
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=DFE7B94EB76B8F26135C9890832CEF6A&aid=3EF0934D8C481DFAA366A47AAD812D1E&yid=67289AFF6305E306&vid=9971A5E270697F23&iid=E158A972A605785F&sid=9CF7A0430CBB2DFD&eid=13553B2D12F347E8&journal_id=1000-3096&journal_name=海洋科学&referenced_num=3&reference_num=34