Solexa sequencing and analysis of the intestinal microorganisms metagenome in Litopenaeus vannamei
凡纳滨对虾肠道微生物宏基因组Solexa 测序及其初步分析

The intestinal microorganisms metagenome of Litopenaeus vannamei was extracted by CTAB-phenol/ chloroform DNA extraction protocol. The relative concentration of bacterial genome in metagenome was analyzed by semi-quantitative PCR. The results showed that the total concentration of metagenome was 92.5 mg/L and relative microbial metagenome ratio was 69.9%. Solexa sequencing showed that up to 64.1% of contigs in metagenome had no assignment or no hits in database of NCBI, 35.5% of which were assigned to eukaryon, and only 0.4% sequences belonged to bacteria and virus.