%0 Journal Article
%T Solexa sequencing and analysis of the intestinal microorganisms metagenome in Litopenaeus vannamei<br>凡纳滨对虾肠道微生物宏基因组Solexa 测序及其初步分析
%A 严雪平
%A 袁剑波
%A 刘 斌
%J 海洋科学
%D 2012
%I 
%X The intestinal microorganisms metagenome of Litopenaeus vannamei was extracted by CTAB-phenol/ chloroform DNA extraction protocol. The relative concentration of bacterial genome in metagenome was analyzed by semi-quantitative PCR. The results showed that the total concentration of metagenome was 92.5 mg/L and relative microbial metagenome ratio was 69.9%. Solexa sequencing showed that up to 64.1% of contigs in metagenome had no assignment or no hits in database of NCBI, 35.5% of which were assigned to eukaryon, and only 0.4% sequences belonged to bacteria and virus.
%K intestinal microfloar metagenome
%K CTAB
%K solexa sequencing<br>肠道微生物宏基因组
%K CTAB
%K Solexa
%K 测序
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=DFE7B94EB76B8F26135C9890832CEF6A&aid=22653CDAF59BBDC1E7E2B728269450AE&yid=99E9153A83D4CB11&iid=B31275AF3241DB2D&sid=9CF7A0430CBB2DFD&eid=F3583C8E78166B9E&journal_id=1000-3096&journal_name=海洋科学&referenced_num=0&reference_num=0