Application of in situ hybridization in testing orange-sported grouper nervous necrosis virus
原位杂交技术在斜带石斑鱼神经坏死病毒检测中的应用

According to the conserved region of the major capsid protein gene of orange-spotted grouper(Epinephelus coioids) nervous necrosis virus(OGNNV),a pair of primers was designed.Using the primers,a RT-PCR product of 426 bp was cloned from the RNA template extracted from orange-spotted grouper infected by OGNNV.The RT-PCR product labeled by digoxigenin(DIG) was used for the non-radioactive nucleic acid probe.A group of orange-spotted groupers were artificialiy infected by injecting infected tissue filtrates and...