%0 Journal Article
%T Application of in situ hybridization in testing orange-sported grouper nervous necrosis virus<br>原位杂交技术在斜带石斑鱼神经坏死病毒检测中的应用
%A CHEN Xiao-yan
%A HE Jian-guo
%A <br>陈晓艳
%A 何建国
%J 海洋科学
%D 2008
%I 
%X According to the conserved region of the major capsid protein gene of orange-spotted grouper(Epinephelus coioids) nervous necrosis virus(OGNNV),a pair of primers was designed.Using the primers,a RT-PCR product of 426 bp was cloned from the RNA template extracted from orange-spotted grouper infected by OGNNV.The RT-PCR product labeled by digoxigenin(DIG) was used for the non-radioactive nucleic acid probe.A group of orange-spotted groupers were artificialiy infected by injecting infected tissue filtrates and...
%K nervous necrosis virus
%K DIG
%K in situ hybridization<br>神经坏死病毒
%K 地高辛
%K 原位杂交
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=DFE7B94EB76B8F26135C9890832CEF6A&aid=EC27BFF9FB1A3EAFE969AB108F9F4EC2&yid=67289AFF6305E306&vid=9971A5E270697F23&iid=B31275AF3241DB2D&sid=CA4FD0336C81A37A&eid=E158A972A605785F&journal_id=1000-3096&journal_name=海洋科学&referenced_num=2&reference_num=9