Adaptability of Litopenaeus vannamei to carbonate saline-alka-line waters in northeast China
凡纳滨对虾对东北碳酸盐型盐碱水域的适应能力

In vitro experiments the antiproliferation activities of polysaccharides from Spirulina platensis(PSP) were evaluated on Hela cells and HepG2 cells by MTT assay,the results showed that PSP(2.5~40 mg/L,48~72 h) exhibited dose and time inhibition effects on the cell growth.The early apoptosis of Hela cells was detected by flow cytometry analysis with a combination of Annexin V-FITC and PI double staining,the apoptosis rates induced by PSP(5,10,20,40 mg/L) were 30.3%,32.6%,34.8% and 38.8% respectively,which were higher than those of the normal control group(P<0.05).Meanwhile,the apoptosis rate treated by PSP(40 mg/L) also increased in time-dependent manner.These results suggested that one of the anti-tumor mechanisms of PSP may be related to inhibition on cell proliferation and induction of notable apoptosis.