%0 Journal Article
%T Adaptability of Litopenaeus vannamei to carbonate saline-alka-line waters in northeast China<br>凡纳滨对虾对东北碳酸盐型盐碱水域的适应能力
%A YANG Fu-yi
%A LI Xiu-jun
%A YANG Xin-qiao
%A SUN Li-min
%A <br>杨富亿
%A 李秀军
%A 杨欣乔
%A 孙丽敏
%J 海洋科学
%D 2008
%I 
%X In vitro experiments the antiproliferation activities of polysaccharides from Spirulina platensis(PSP) were evaluated on Hela cells and HepG2 cells by MTT assay,the results showed that PSP(2.5~40 mg/L,48~72 h) exhibited dose and time inhibition effects on the cell growth.The early apoptosis of Hela cells was detected by flow cytometry analysis with a combination of Annexin V-FITC and PI double staining,the apoptosis rates induced by PSP(5,10,20,40 mg/L) were 30.3%,32.6%,34.8% and 38.8% respectively,which were higher than those of the normal control group(P<0.05).Meanwhile,the apoptosis rate treated by PSP(40 mg/L) also increased in time-dependent manner.These results suggested that one of the anti-tumor mechanisms of PSP may be related to inhibition on cell proliferation and induction of notable apoptosis.
%K polysaccharides from Spirulina platensis(PSP)
%K antitumor effect
%K apoptosis
%K annexin V/PI<br>钝顶螺旋藻多糖
%K 抗肿瘤作用
%K 细胞凋亡
%K AnnexinV/PI
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=DFE7B94EB76B8F26135C9890832CEF6A&aid=3E56E1A827E8A3DC9B93942DCD30C8D6&yid=67289AFF6305E306&vid=9971A5E270697F23&iid=CA4FD0336C81A37A&sid=2001E0D53B7B80EC&eid=1AE5323881A5ECDC&journal_id=1000-3096&journal_name=海洋科学&referenced_num=0&reference_num=13