Cloning and analyse of nucleoside diphosphate kinase gene sequence from marine polyphosphate-accumulating bacterium, Halomonas YSR-3
海洋聚磷菌中核苷二磷酸激酶基因的克隆及序列分析

Nucleoside diphosphate kinase(NDPK) gene was amplified from marine polyphosphate-accumula- ting bacterium Halomonas YSR-3 by PCR and molecular clone techniques and inserted into a pGM-T vector.The recombinant plasmid was transformed into an Escherichia coli TOP10 strain.The positive transformants with NDPK gene were obtained by blue-white selection and clony PCR method,then the positive ones were sequenced. The sequenced fragment concluded 420 pairs of bases and was analyzed by NCBI database.The similaritie...