%0 Journal Article
%T Cloning and analyse of nucleoside diphosphate kinase gene sequence from marine polyphosphate-accumulating bacterium, Halomonas YSR-3<br>海洋聚磷菌中核苷二磷酸激酶基因的克隆及序列分析
%A REN Shi-ying
%A XIAO Tian
%A <br>任世英
%A 肖天
%J 海洋科学
%D 2008
%I 
%X Nucleoside diphosphate kinase(NDPK) gene was amplified from marine polyphosphate-accumula- ting bacterium Halomonas YSR-3 by PCR and molecular clone techniques and inserted into a pGM-T vector.The recombinant plasmid was transformed into an Escherichia coli TOP10 strain.The positive transformants with NDPK gene were obtained by blue-white selection and clony PCR method,then the positive ones were sequenced. The sequenced fragment concluded 420 pairs of bases and was analyzed by NCBI database.The similaritie...
%K Halomonas
%K polyphosphate-accumulating bacterium
%K nucleoside diphosphate kinase(NDPK)<br>盐单胞菌属
%K 聚磷菌
%K 核苷二磷酸激酶（NDPK）
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=E62459D214FD64A3C8082E4ED1ABABED5711027BBBDDD35B&cid=2D9C75573FA3E416&jid=DFE7B94EB76B8F26135C9890832CEF6A&aid=34B86E3D1DAD59CA06D4B01FC48F8677&yid=67289AFF6305E306&vid=9971A5E270697F23&iid=9CF7A0430CBB2DFD&sid=1D0FA33DA02ABACD&eid=E84BBBDDD74F497C&journal_id=1000-3096&journal_name=海洋科学&referenced_num=0&reference_num=11